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Complete Genome Sequence and Genome Analysis of Eggplant mottled dwarf virus-Iranian Isolate
Ghobad Babaie, Mina Kouhi Habibi, Amir Massah, Akbar Dizadji, Laleh Izadinejad and Anne Simon. 2015. Complete Genome Sequence and Genome Analysis of Eggplant mottled dwarf virus-Iranian Isolate. doi: 10.1111/jph.12256. J. Phytopathology,163:331-341.
Abstract
The full-length nucleotide sequence of the Iranian isolate of Eggplant mottled
dwarf virus (EMDV), a phytorhabdovirus, was determined using the
random polymerase chain reaction method (rPCR) followed by PCR with
specific primers to fill in the gaps. The negative-sense RNA genome of the
Iranian isolate of EMDV contains 13154 nucleotides and seven open-reading
frames (ORFs) in the order 30-leader-N-X-P-Y-M-G-L-trailer-50. These
ORFs encode the nucleocapsid, X protein (of unknown function), phosphoprotein,
Y protein (putative movement protein), matrix protein,
glycoprotein and RNA-dependent RNA polymerase, respectively. EMDV
has a 199 nt 30 leader RNA and a 151 nt 50 trailer, and the ORFs are separated
by conserved intergenic sequences. Phylogenetic analyses indicate
that EMDV is most closely related to Potato yellow dwarf virus, which has a
distinctly different geographical distribution.
Journal Papers
Month/Season:
Spring
Year:
2015